Scientific Imaging with Photoshop by Jerry Sedgewick

Author:Jerry Sedgewick
Language: eng
Format: epub
Publisher: Pearson Education Limited (US titles)
Published: 2008-03-17T04:00:00+00:00

Note

In CS3, be sure to select the Legacy check box.

Hold down Shift and use the Move tool to drag the flatfield image over the image of the specimen; or select all, copy, and paste. This will put the flatfield image on a layer above the specimen image.

Invert the flatfield image layer by selecting Image > Adjustments > Invert (Figure 6.7C). The flatfield layer should be pure gray. If not, desaturate the flatfield image by selecting Image > Adjustments > Desaturate.

In the Layers palette, choose Hard Light from the Blend Mode menu (Figure 6.7D).

Adjust opacity to 50%, or until the color samplers are at or near the same reading (depending on your confidence at having chosen similarly bright sampling points for the specimen image in step 1).

Flatten the image (Layers > Flatten or choose Flatten Image from the drop-down menu in the Layers palette) to complete flatfield correction.

The image will be darker than it was when the process began. Move the sampling points to the whitest, significant area and to the approximate darkest part of the image (Figure 6.7E). Using Levels (Figure 6.7F), set the black and white limits (see the section “Standard Procedure” in Chapter 5).

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