Psilocybin Mushroom Handbook by G Nicholas

Author:G Nicholas
Language: eng
Format: epub
Publisher: Quick Trading Company
Published: 2010-08-11T04:00:00+00:00

Retrieving Cultures From Storage

To retrieve a culture from its storage container, return the culture to room temperature for 48 hours, and transfer (under the usual sterile conditions) a small chunk of mycelium-covered paper from the storage container to a fresh, peroxidated agar Petri dish.

8

WORKING WITH GRAIN

Grain Spawn Preparation

Grain spawn is made of cooked whole grains with small amounts of calcium carbonate and calcium sulfate added. The calcium carbonate serves as a pH buffer, while the calcium sulfate keeps the individual grain kernels from sticking together; both provide mineral nutrition to the fungus. To ensure even cooking and thorough sterilization, the grain is boiled briefly, soaked overnight in hot water, then drained, loaded into containers, and sterilized.

Many grains can harbor bacterial endospores. These highly resistant structures are designed to withstand extreme environmental conditions, allowing them to survive sterilization. Soaking the grain overnight forces these endospores to germinate, at which point heat will easily kill the live bacteria.

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