Practical Guide to Diagnostic Parasitology by Lynne Shore Garcia

Author:Lynne Shore Garcia
Language: eng
Format: epub, pdf
ISBN: 9781683673507
Publisher: Wiley
Published: 2009-11-14T16:00:00+00:00

Diagnostic Methods

Direct Wet Examinations

1. What is the purpose of the direct wet examination?

The direct wet examination is designed to allow the viewer to detect motile protozoa; this procedure should not be performed on preserved specimens and should be reserved for fresh stool specimens that are very soft or liquid. Organism identification is often presumptive; permanent stained smears must also be examined.

2. How should the direct wet preparation be examined?

The entire coverslip preparation (22 by 22 mm) should be examined under low magnification (×100); approximately one-third to one-half of the coverslip preparation should be examined under high dry magnification (×400). It is not practical to examine this preparation using oil immersion magnification (× 1,000). Saline and/or iodine mounts can be examined; however, iodine kills any organisms present, so trophozoite motility will no longer be visible.

3. What do you expect to see during a wet preparation examination?

Helminth eggs and/or larvae can be seen, as well as some protozoan cysts, white blood cells (WBCs), some yeast, and fecal debris. Many of the intestinal protozoa will need to be confirmed using the oil immersion magnification (×1,000) for the permanent stained smear.

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