Immunohistochemistry by Alexander E. Kalyuzhny

Author:Alexander E. Kalyuzhny
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

Fig. 5.4Effects of heat-induced epitope retrieval (HIER) on the intensity of IHC staining. Without HIER (a), antigen labeling is much weaker compared with HIER-treated tissue (b). Tissue sections were stained using HRP-DAB detection (brown color) and counterstained with hematoxylin (blue color)

5.2 Mounting Media

Mounting media is an essential reagent in IHC. After IHC staining is completed, stained tissue sections need to be protected from damage during their examination under the microscope and during storage. Tissue sections are covered with very thin pieces of glass called coverslips that, in addition to providing protection, also serve as an observation window. Before coverslips are placed on the tissue section they must be wet with few drops of mounting media, which serves as a glue for coverslips (Fig. 5.5).

Fig. 5.5Schematic overview of covering stained tissue sections with glass coverslips

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.