Correlative Imaging by Unknown

Author:Unknown
Language: eng
Format: epub
Publisher: John Wiley & Sons, Incorporated
Published: 2019-10-21T00:00:00+00:00

Figure 6.7 In situ correlated SEM/AFM imaging of a collagen lined lacunae in bovine trabecular bone. a: AFM integrated inside the SEM allows for direct, high depth of focus viewing of both tip and sample. b: using the large field of view and fast image acquisition of the SEM it is possible to find rare and small features like lacunae and the AFM cantilever can be positioned there even on very irregular surfaces. c: AFM overview image of the lacunae showing the collagen fibers. d: High‐resolution image of the collagen fibers showing the characteristic 67 nm periodic banding pattern. e: X‐Z cross section of the banding pattern revealing a corrugation height of 3 nm.

Images were taken using the AFSEMTM instrument (GETec GesmbH) integrated in a Quanta field emission SEM (FEI). Image courtesy Dr. Marcel Winhold.

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