Antigen Processing by Peter Endert

Author:Peter Endert
Language: eng
Format: epub
Publisher: Humana Press, Totowa, NJ

(c)Most laboratories further supplement the culture medium with mitogenic compounds or conditioned medium see Note 4 and Subheading 3.2.5.

(d)Most laboratories feed be-weekly the wells exchanging half the volume of RPMI human complete medium supplemented with human IL-2 with on day 14 addition of 105 irradiated (20 Gy) ideally autologous PBMC.

3.2.4 Epstein-Barr Virus (EBV) Immortalization of B Lymphocytes (8)

1.Prepare supernatant containing EBV by transferring exponentially growing B95.8 cells (1  ×  106/ml) in fresh RPMI 1640 medium supplemented with 10% FCS 100 U/ml Penicillin, 100 μg/ml Streptomycin (RPMI complete medium).

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