Skeletal Muscle Regeneration in the Mouse by Michael Kyba
Author:Michael Kyba
Language: eng
Format: epub
Publisher: Springer New York, New York, NY
11.Scrape the muscle solution against the bottom of the inclined petri dish and squeeze into and out of a sheared Pasteur pipette (with small bulb). This will mechanically disrupt the muscle fibers .
12.Add 2 mL rinsing solution to wash the petri dish, transferring the solution back to the 50 mL conical tube. Repeat this rinse to collect as much residue as possible.
13.Centrifuge the samples at 300 × g for 5 min on a benchtop centrifuge.
14.Aspirate the supernatant, leaving behind 5 mL.
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