Neuroanatomy and Neurophysiology of the Larynx by Yasuo Hisa

Author:Yasuo Hisa
Language: eng
Format: epub
Publisher: Springer Japan, Tokyo

6.7 Involvement of Neuropeptides in Laryngeal Sensory Innervation [38, 39]

We investigated which neuropeptides play important roles in sensory innervation of the larynx. We labeled nodose ganglion cells that supply the internal branch of the superior laryngeal nerve with sensory nerve fibers, by infusing gold-labeled cholera toxin B (CTBG) into the internal branch of the canine superior laryngeal nerve. After colchicine processing followed by perfusion fixation, nodose ganglia were extirpated, and histological sections were prepared. After visualization of labeled cells by silver enhancement, immunohistochemical analyses were carried out using calcitonin gene-related peptide (CGRP), substance P (SP), and leu-enkephalin (ENK), and the positivity rate of each neuropeptide in CTBG-labeled cells was determined.

Among all labeled cells, the CGRP positivity rate was the highest, 81.5 %, whereas the SP positivity rate was 24.5 %, and the ENK positivity rate was 7.0 % (Fig. 6.3).

Fig. 6.3CTBG was injected into the internal branch, and the SP immunohistochemical method was used to label cells in the nodose ganglion. There were several SP-positive cells among the CTBG-labeled cells [38]

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