Bacterial Cell Wall Homeostasis by Hee-Jeon Hong

Author:Hee-Jeon Hong
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

9.Record and compare RLU values to compare relative CWSS expression and/or induction levels (Fig. 2).

Fig. 2Example of induction curves showing RLU detected from the construct psas016 p-luc+ in S. aureus cultures exposed to different oxacillin concentrations. Oxacillin was added to mid exponential cell cultures and relative light units (RLU) were measured at time points up to 2 h post addition. Concentrations were chosen in relation to the oxacillin minimum inhibitory concentration (MIC) of the strain. Squares—5× MIC, triangles—2× MIC, circles—1× MIC, diamonds—0.5× MIC, and asterisks—no oxacillin

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