Innate Antiviral Immunity by Karen Mossman

Author:Karen Mossman
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

5.Increasing incubation time past 4 h has little to no effect on RNA yield. However, decreasing the temperature to 30 °C can result in higher quality poly-U/UC RNA due to higher fidelity read-through of the uridine tract.

6.We have featured transfection in a 12-well dish here, which should provide adequate cell numbers for most downstream PAMP assessment assays (i.e., immunoblotting , or qPCR). If more cells are needed, reagent volumes and cell numbers should be adjusted according to cell culture dish size used. 80% confluency of cells in culture at the time of transfection is optimal.

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