Biophysics Tools and Techniques by Mark C. Leake

Author:Mark C. Leake
Language: eng
Format: epub
Publisher: CRC Press

6.3.4 APPLICATIONS OF OPTICAL TWEEZERS

Appropriate latex or silica-based microspheres suitable for optical trapping can be commercially engineered to include a chemical coating of a variety of different compounds, most importantly carboxyl, amino, and aldehyde groups that can be used as adapter molecules to conjugate to biomolecules. Using standard bulk conjugation chemistry, these chemical groups on the bead surface can be bound either directly to biomolecules or more commonly linked to an adapter molecule such as a specific antibody or a biotin group that will then bind to a specific region of a biomolecule of interest (see Chapter 7). Chemically functionalizing microspheres in this way allows single biomolecules to be attached to the surface of an optically trapped bead and tethered to a fixed surface such as a microscope coverslip (Figure 6.3a).

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.