Wheat Biotechnology by Prem L. Bhalla & Mohan B. Singh

Author:Prem L. Bhalla & Mohan B. Singh
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

2.The binary vector pGH215 harbors the HYGROMYCIN PHOSPHOTRANSFERASE (Hpt) selectable marker gene, driven by the doubled-enhanced CaMV 35S promoter; the sGfp (S65T) reporter gene, driven by the maize UBIQUITIN-1 promoter including its first intron; and T-DNA borders derived from a nopaline Ti plasmid [13] (see Note 2). The binary vector was introduced into AGL1 by electroporation. Derived vectors carrying an intron in the Hpt-coding sequence have also been used, rendering the bacterium fully susceptible to the selective agent; as a result, treatment with hygromycin highly effectively prevents the persistence of Agrobacterium after cocultivation.

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.