Western Blotting Guru by Ayaz Najafov Gerta Hoxhaj & Gerta Hoxhaj
Author:Ayaz Najafov,Gerta Hoxhaj & Gerta Hoxhaj [Najafov, Ayaz & Hoxhaj, Gerta]
Language: eng
Format: epub
ISBN: 9780128135389
Publisher: Elsevier B.V.
Published: 2017-03-15T00:00:00+00:00
Proteins were over-transferred For small proteins, this can be a problem. Decrease transfer time to 0.5 h at 100 V. Use membranes with smaller pore size. Use gels with higher concentrations
Ponceau S stock is old or has been reused too many times Prepare fresh Ponceau S stock
Proteins were degraded and/or dephosphorylated prior to loading Store protein samples in SDS–PAGE sample buffer at −20°C and avoid repeated freeze–thaw cycles. Prepare fresh samples. Do not leave samples on bench top for prolonged time periods. Do not heat protein samples for longer than 10 min
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