The Handbook of Plant Metabolomics by Wolfram Weckwerth
Author:Wolfram Weckwerth
Language: eng
Format: epub
Publisher: Wiley
Published: 2013-02-18T16:00:00+00:00
10.2.3 Preparation of Biological Material with Biotechnological Methods (Callus, Cell, or Hairy Root Cultures)
Biological material such as cell cultures, callus, and hairy root cultures should be grown under defined conditions (temperature, humidity, light) and culture medium composition (nutrients, pH). Samples should be collected in a proper number of biological and technical repetitions; also, media of the investigated cultures should be collected for further analyses. The conditions of collection and storage of plant cell and tissue cultures should follow the procedure described below.
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