The Bacterial Nucleoid by Olivier Espéli

Author:Olivier Espéli
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

Before each experiment, follow these steps:1.Rinse the glass channel with deionized H2O and sonicate for 15 min.

2.A functionalized #0 coverslip is then sandwiched in between #1.5 coverslips and Parafilm (see Fig. 4b), with the #0 functionalized coverslip protruding out slightly (~1 mm).

3.Heat the stack of coverslips on a hot plate (~150 °C) to melt the Parafilm and stick the coverslips together.

4.Insert the stack of coverslips into the glass channel (see Note 3 ) and seal the gap with wax (melt at ~150 °C before use).

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