Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants by Shri Mohan Jain & Pramod Gupta

Author:Shri Mohan Jain & Pramod Gupta
Language: eng
Format: epub
Publisher: Springer International Publishing, Cham

16.2.4 Germination and Conversion of Somatic Embryos

1. Transfer the somatic embryos to the Germination culture medium (Table 16.2) under the same environmental conditions (temperature and photoperiod). Somatic embryos germinate normally (Fig. 16.5). Separate somatic embryos from the callus without any further delay for preventing formation of secondary SE.

Fig. 16.5Embling conversion from SE

1.In order to obtain plants, place the germinated somatic embryos in Petri dishes (60 × 15 mm) with sterile vermiculite, moist with culture liquid half strength MS medium or on bridge filter paper in test tubes filled with 25 ml same culture medium .

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