Placenta and Trophoblast by Michael J. Soares & Joan S. Hunt

Author:Michael J. Soares & Joan S. Hunt
Language: eng
Format: epub
Publisher: Humana Press, Totowa, NJ

2 Materials

1.The BeWo cell line was originally derived from a human choriocarcinoma. The BeWo clone (b30) in use in our laboratory was obtained from Dr. Alan Schwartz (Washington University, St. Louis, MO). The original BeWo cell line is also available through American Type Culture Collection (ATCC; Manassas, VA, cat. no. CCL-98); however, it does not have the same monolayer-forming behavior in culture as the Schwartz clone.

2.Cell culture medium. This is prepared using Dulbecco’s modified Eagle’s medium (DMEM) (with L-glutamine and 4500 mg glucose/L, without sodium bicarbonate; Sigma Chemical Co., St. Louis, MO, cat. no. D-5648), heat-inactivated fetal bovine serum (FBS) (Atlanta Biologicals, Norcross, GA), penicillin/streptomycin (10,000 U/mL; Invitrogen, Carlsbad, CA), and MEM nonessential amino acids (Invitrogen).

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