Japanese Marine Life by Unknown
Author:Unknown
Language: eng
Format: epub
ISBN: 9789811513268
Publisher: Springer Singapore
Material and Methods
Material
Mytilus galloprovincialis
Solutions
ASW (530 mM NaCl, 13.3 mM KCl, 12.6 mM MgCl2, 19.5 mM CaCl2, 2.6 mM NaHCO3, pH 8.0)
10−5 M acetylcholine (ACh) in ASW
10−5 M 5-hydroxytryptamine (5-HT, serotonin) in ASW
Instruments
Isotonic lever transducer with amplifier (we used a lab-made transducer and amplifier, but commercial devices, such as the TD-111 T by Nihon Kohden, HSE-HA by Harvard Apparatus, and MLT7006 by ADInstruments will suffice)
Recorder (we used the Thermal Arraycorder by Graphtec, but any digital or paper chart recorder will suffice)
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