Immobilization of Enzymes and Cells by Jose M. Guisan

Author:Jose M. Guisan
Language: eng
Format: epub
Publisher: Humana Press, Totowa, NJ

6.3.1 Methods

1.Seed 5 × 103 cell per well in a 96 well plate at 37 °C and 5 % CO2 in cell culture media with a final volume of 100 μL.

2.After 24 h, the particles were filtered using a 0.2 μm syringe filter to sterilize the particles. Then, dilutions in fresh media of the particles in a range of concentration were prepared. The old media replaces the NPs dilutions. At least three replicates per concentration of NPs and per NPs type are required in order to carry out a comprehensive study to obtain appropriate statistics.

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