Epigenome Editing by Albert Jeltsch & Marianne G. Rots
Author:Albert Jeltsch & Marianne G. Rots
Language: eng
Format: epub
Publisher: Springer New York, New York, NY
4.NorthernMax 10× MOPS running buffer (3-(N-morpholino)propanesulfonic acid, Ambion, Austin, USA).
5.RNA formaldehyde gel. Add 1 g of agarose to 75 ml DEPC-treated water, boil in the microwave, and then cool down the solution to 60 °C. Add 18 ml of formaldehyde solution (Sigma, St. Louis, USA) and then 10 ml of 10× MOPS, and immediately pour the mixture into a gel cast. Leave the gel cooling for 1 h (see Note 1). Allow to run in 1× MOPS running buffer made with Millipore water.
6.ssRNA ladder (New England Biolabs, Ipswich, USA).
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