DNA and RNA Isolation Techniques for Non-Experts by Akash Gautam

Author:Akash Gautam
Language: eng
Format: epub
ISBN: 9783030942304
Publisher: Springer International Publishing

7.

Centrifuge at 12,000×g for 10 min at room temperature (RT).

8.

Transfer the aqueous phase into a fresh tube using a wide-bore pipette (Fig. 1).

9.

Repeat steps 4–6 till the aqueous layer becomes clear.

10.

Measure the sample volume and add volume of chilled isopropanol (−20 °C). Mix gently by inversion and let the DNA precipitate for 30 min at −20 °C (Fig. 2).

11.

If DNA has precipitated nicely, spool it out (Fig. 3) and transfer into a 2 mL microcentrifuge tube, else centrifuge at 10,000 rpm for 10 min at RT and decant the supernatant very carefully.

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.