Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres by Yusuke Kawamoto

Author:Yusuke Kawamoto
Language: eng
Format: epub
ISBN: 9789811369124
Publisher: Springer Singapore

Next, following the previous report [12], we used immunofluorescence to prove that the TH59TR and Deamino TH59TR can localize to telomeres. We reasoned that a shelterin complex component in telomeres, TRF1 protein, which binds to TTAGGG repeats and controls telomere length [2–5], should colocalize with the TH59TR and Deamino TH59TR. Figure 2.4 shows human HeLa cells treated with the TH59TR and Deamino TH59TR (red) together with the immunofluorescence signal of TRF1 antibodies (green). This examination suggested that the signals of TH59TR and Deamino TH59TR were colocalized with that of TRF1 because the resulting overlapping yellow signals were observed.

Fig. 2.4Staining of telomeric repeats with fluorescent polyamides and co-localization with telomere binding protein TRF1. HeLa1.3 cells were stained with DAPI, TRF1 antibody and TH59TR (a) or Deamino TH59TR (b). Enlarged images of the boxed regions in 1st row images are shown on 2nd row. Note that TH59TR or Deamino TH59TR foci co-localize with immunofluorescence signals with TRF1 antibodies.

Copyright 2013 American Chemical Society

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