Order and Fluctuations in Collective Dynamics of Swimming Bacteria by Daiki Nishiguchi

Author:Daiki Nishiguchi
Language: eng
Format: epub
ISBN: 9789813299986
Publisher: Springer Singapore

(3.1)

Thus we obtained intensity-calibrated images (Fig. 3.13c).

To ensure our analysis methods, we applied the same image processing procedures and the same analysis to both the ordered phase (Fig. 3.8d) and the disordered phase (Fig. 3.14).

Fig. 3.13a Raw image after the dark current subtraction. Intensity is adjusted in the range [0, 1] and contrast is enhanced for visibility. This image corresponds to the numerator in Eq. (3.1). b Intensity distribution of excitation light source for fluorescent microscopy. Fluorescence intensity of a homogeneous fluorophore solution was obtained by the 12-bit camera and then after dark current subtraction it is used as the denominator of Eq. (3.1). c Calibrated image. Intensity is adjusted in the range [0, 1] and contrast is enhanced for visibility. d Binarized image used for analysis on number fluctuations

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