Molecular Dermatology by Unknown

Molecular Dermatology by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781071606483
Publisher: Springer US


4 Notes

1.William’s E culture medium can be reused if stored at +4 °C. It is important to carefully check the color of the medium because it can be reused until the color remains red.

2.We use recombinant IFNγ (MW 16879.29 g/mol) expressed in E. coli without any modifications for all our experiments. Prepare 2000 and 20 IU/μL stock solutions of IFNγ in DPBS 0.1% BSA and store at −20 °C upon use. Avoid repeated freeze–thaw cycles. Dilute 20 IU/μL stock solutions of IFNγ in WCM accordingly to obtain 75 IU/mL concentrations.



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