Microbial Resource Conservation by Sushil K. Sharma & Ajit Varma

Author:Sushil K. Sharma & Ajit Varma
Language: eng
Format: epub
ISBN: 9783319969718
Publisher: Springer International Publishing

Remove an agar block from a working stock, and inoculate on a suitable agar medium. Alternatively, organism can be recovered by transferring a small amount of the suspension onto suitable agar medium and incubating under optimum growth conditions. After the initiation of growth, the culture may be monitored for viability and contamination and then may be used.

8.9 Preservation/Storage in Mineral Oil

Mineral oil (paraffin oil) has been traditionally used as an effective method of long-term preservation of live cultures (Lumiere and Chevrotier 1914). Several modifications are found in literature to suit the specific requirement of a particular isolate. This method has been well recognized for preserving diverse group of microbes, and economics were also studied and reaffirmed. In principle sterile mineral oil prevents desiccation and is reported to diminish gas exchange which substantially reduces the metabolism of fungal strains to be stored. By applying this method, culture(s) can be maintained for years together. This method is space intensive and needs periodic inspection for contaminations. This method has been reviewed in detail, and the protocol provided here is as per Singh (2017).

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