Metabolic Profiling by Georgios A. Theodoridis Helen G. Gika & Ian D. Wilson

Author:Georgios A. Theodoridis, Helen G. Gika & Ian D. Wilson
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

10.Repeat centrifugation as many times as required until no precipitate is visible.

11.Transfer all the supernatant into a high-recovery autosampler glass vial (if the supernatant volume is small), or, if possible, divide it into 2–4 replicates.

12.Vacuum dry the supernatant samples .

13.Cap each vial with a PTFE crimp cap and store at 4 °C until further analysis.

Blood Plasma or Other Liquid (e.g., Culture Medium) Samples

1.Add 200 μL of blood plasma into a 2 mL Eppendorf tube containing 0.6 mL of ice-cold methanol and 2 μg of adonitol/ribitol and 4 μg of U-13C-d-glucose as internal standards (see Note 3 ).

Download

Copyright Disclaimer:
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.