Medical Retina by Frank G. Holz & Richard Spaide
Author:Frank G. Holz & Richard Spaide
Language: eng
Format: epub
Publisher: Springer Berlin Heidelberg, Berlin, Heidelberg
The transmission of ocular media hinders the excitation of fluorophores in their maxima below 400 nm.
Several fluorophores emit a weak fluorescence below 560 nm.
The fundus fluorescence above 560 nm is dominated by lipofuscin.
Lifetime measurements are independent of fluorophore concentration and absorption into the surrounding tissue.
Lifetime measurements permit the discrimination of a weakly emitting fluorophore whose emission spectrum is covered by a strongly emitting fluorophore.
A fluorescence lifetime mapping ophthalmoscope permits excitation at different wavelengths and time-resolved fluorescence measurements of two spectral ranges (490–560 nm, 560–700 nm).
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