Genotoxicity Assessment by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781493996469
Publisher: Springer New York

10. Hen’s Egg Test for Micronucleus Induction (HET-MN)

Kerstin Reisinger1 , Eva Dony2, Thorsten Wolf3 and Katrin Maul4

(1)Henkel AG & Co. KGaA, Düsseldorf, Germany

(2)Envigo CRS GmbH, Rossdorf, Germany

(3)Institute for Health Research and Education, University of Osnabrueck, Osnabrück, Germany

(4)Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany

Kerstin Reisinger

Email: [email protected]

Abstract

The classical in vitro genotoxicity test battery is known to be sensitive for indicating genotoxicity. However, a high rate of “misleading” positives was reported when three assays were combined as required by several legislations. Despite the recent optimizations of the standard in vitro tests, two gaps could merely be addressed with assays based on monolayer cell cultures, that is, the route of exposure and a relevant intrinsic metabolic capacity to transform chemicals into reactive metabolites. Following these considerations, fertilized chicken eggs have been introduced into genotoxicity testing and were combined with a classical readout parameter, i.e., the analysis of micronucleus frequency in erythrocytes, to develop the hen’s egg test for micronucleus induction, the HET-MN. As a major advantage the test mirrors the systemic availability of compounds after oral exposure reflecting certain steps of ADME without being considered as an animal experiment. After a successful validation exercise the detailed protocol is given here.

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