G-Quadruplex DNA by Peter Baumann

Author:Peter Baumann
Language: eng
Format: epub
Publisher: Humana Press, Totowa, NJ

8.1.4 Stern–Volmer Quenching

Classical Stern–Volmer quenching (also referred to as dynamic quenching) can be explained by a simple mechanism in which the quenching agent Q collides with an excited fluorophore F*. In this type of quenching, the excited state energy is lost when the excited fluorophore collides with Q and forms a transient encounter complex as depicted in Eq. 8.1:

(8.1)

Formation of (F*⋅⋅⋅⋅⋅Q) depends on the diffusion rate constant for F* and Q, their concentrations, and the efficiency of the quenching process. For a single fluorescent species in a homogeneous environment, the degree of quenching increases with increasing [Q] according to the Stern–Volmer relationship:

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