Developing Novel Spinning Methods to Fabricate Continuous Multifunctional Fibres for Bioapplications by Azadeh Mirabedini

Author:Azadeh Mirabedini
Language: eng
Format: epub, pdf
ISBN: 9783319953786
Publisher: Springer International Publishing

3.2 Experimental

3.2.1 Materials

Medium molecular weight chitosan was used in this study as the core spinning solution. Toluidine Blue O (also known as toluidine blue or TB) as an indicator ingredient was supplied from Sigma-Aldrich Co. for the release experiments.

A simulated body fluid (SBF) solution, with ion concentrations approximately equal to those of human blood plasma, was prepared with the following composition; 142 mM Na+, 5 mM K+, 1.5 mM Mg2+, 2.5 mM Ca2+, 103 mM Cl−, 27 mM , 1.0 mM and 0.5 mM with the final adjusted pH of 7.4 ± 0.05 [26]. This solution was used as an aqueous medium to re-swell the dried fibres for imaging.

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