Clinical Epigenetics by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9789811389580
Publisher: Springer Singapore

5.8 Future Requirements

CircRNAs show tremendous potential as clinical biomarkers of disease diagnosis, prognosis and treatment assessment. Encouraging circRNA biomarker usage is the development of new circRNA detection tools to identify previously undiscovered circRNA types. One promising detection tool is accurate circRNA_finder suite (acfs), the first circRNA detection tool to identify fusion circRNAs (You and Conrad 2016). To further progress the use of circRNA biomarkers, the establishment of reputable pipelines with high sensitivity and precision is essential. For such pipelines, performance standards should be comparable to current pipelines available in linear RNA-seq analysis. In the meantime, multi-bioinformatic approaches should be adopted. Also, spurring the use of circRNA biomarkers is the introduction of third-generation sequencing, which enables long-read sequencing and direct RNA-seq. Implementing third-generation sequencing removes artefacts and bias which would arise from cDNA conversion and simplifies computational analysis by generating full-length reads. What’s more is that direct RNA-seq technologies provides real time, massive parallel sequencing, with portability and affordability. Taken together with data produced to date, it is highly likely that the discovery of more disease-driving circRNAs will occur in the future, which opens the door to their routine clinical use as early detection biomarkers, prognostic indicators and therapeutic efficacy indicators.

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