Kidney Research by Tim D. Hewitson Edward R. Smith & Stephen G. Holt

Author:Tim D. Hewitson, Edward R. Smith & Stephen G. Holt
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

Probe

Characteristic

Function

2-Photon ex range

Nuclear and cell structure

Hoechst 33342

Labels cell nuclei blue

Nuclear change (apoptosis, mitosis)

780–820 nm

Propidium iodide

Labels cell nuclei red, membrane impermeable

Necrotic change of nuclei

820–850 nm

Calcein-acetoxymethyl (AM)

Labels cytosol of tubular and endothelial cells

Cell viability

800 nm

Quinacrine

Loads preferentially into brush border and subapical vesicles

Endo- and exocytosis, and apical shedding. Renin granules in the juxtaglomerular apparatus

700–720 nm

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