Disease Gene Identification by Johanna K. DiStefano

Author:Johanna K. DiStefano
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

17.Place in thermal cycler and run the Library Amplification protocol.

18.This is a safe stopping point where samples can be stored at 4 °C for up to 2 days.

Fig. 8Nextera® XT dual indexing set up. Nextera® XT index set up using Illumina’s TruSeq Index Plate Fixture. Index 1 (i7) goes across the top of the plate (orange caps) while Index 2 (i5) goes vertically down the side (white caps). Add i7 indexes down each column and add i5 indexes across each row. If making less than 12 libraries please see the Illumina low plexing guidelines

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