Behavior of Radionuclides in the Environment I by Kenji Kato & Alexei Konoplev & Stepan N. Kalmykov

Author:Kenji Kato & Alexei Konoplev & Stepan N. Kalmykov
Language: eng
Format: epub
ISBN: 9789811506796
Publisher: Springer Singapore

Enzyme

Concentration

Temp. & Time

HB FA(%)

Lysozyme

10, 20 or 30 mg mL−1

37°C, 60 min

35 or 50

Proteinase K

1, 10 or 100 μg mL−1

37°C, 30 min

35 or 50

Zymolyase

1 or 10 mg mL−1

37°C, 60 min

35 or 50

Achromopeptidase

0.0006, 0.006, 0.06, 0.6, 6.0 or 60 U mL−1

37°C, 30 min

35 or 50

Zymolyase + Proteinase K

1 mg mL−1 and 1 μg mL−1

37 °C, 5–60 min and 37 °C, 1–30 min

50

To check whether the employed condition destroys the cell structure of Pseudomonas stutzeri and Paracoccus denitrificans or not, cells permeabilized by each enzyme and non-treatment cells were stained with 4′6-diamidino-2-phenylindole (DAPI, Nacalai.

Tesque; 0.1 μg mL−1) for 7 min and cell number was counted based on pictures taken under a universal epifluorescence microscopy system (BX-51-FLA; Olympus, Tokyo, Japan) with a digital camera (DP-71, Olympus) with an image analysis system (DP controller, Olympus).

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