Practical phsyiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science by Hawk Philip Bovier 1874-

Author:Hawk, Philip Bovier, 1874- [from old catalog]
Language: eng
Format: epub
Tags: Biochemistry
Publisher: Philadelphia, P. Blakiston's son & co
Published: 1918-03-25T05:00:00+00:00

ACIDOSIS

329

it The levelling bulb is then raised in the left hand whUe with the right the cock is turned so as to connect the pipette with f. The mercury flowing in from c fills the body of the pipette, and as much of the calibrated stem at the top as is not occupied by the gas extracted from the solution, A few hundredths of t c,c, of water which could not be completely drained into // float on top of the mercury in the pipette, but the error caused by reabsorption of carbon dioxide into this small volume of water is negligible if the reading is made at once. The mercury bulb Is placed at such a level that the gas in the pipette is under atmos-pheric presstire^ and the volume of the gas is read on the scale.

Calculation.—By means of the table on page 330 the readings on the apparatus can be directly transposed into c.c* of carbon dioxide chemically bound by 100 c«c. of plasma. The barometer reading and room temperature are taken at the time of the determination. For convenience in the calculation values are

B pven below for the ratio , over the range usually encountered.

In case the volume of plasma taken for estimatioii of carbon dioxide content i 0.5 c.c, the observed volume of gas is multiplied by 3 before it is used to ite the volume per cent of carbon dioxide bound.

Interpretation. —The carbon dioxide capacity of the plasma as [determined by this method appears to indicate not only the alkaline ' reser\'e of the blood but also that of the entire body. The average nonnal value for man is 65 volume per cent of carbon dioxide. The table, page 331, shows the range of results obtained with normal and pathological plasma^ as well as the relationship of the plasma bicarbonate to add excretion, alkali tolerance, and alveolar carbon dioxide tension.

* In order to have the column in the pipette exactly balanced by that outside, the surface U the mercury in the levelling bulb snould be raised until it is level with the mercury fteaiflcus in the pipette, and then, for entire accuracy, raised above the latter meniscus fcyt distance equal to J-ij 4 the height of the column of water above the mercury in the P^tte. As the water column is as a nile, only about 10 mm. high, the correction that w to be estimated is less than i mm. of mercury^ i. e.^ the entire correction for the water Goliiiiui ia not enough to InfiueBce results appreciably.

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