Immunosensors by Ahmed Minhaz Uddin;Zourob Mohammed;Tamiya Eiichi;

Author:Ahmed, Minhaz Uddin;Zourob, Mohammed;Tamiya, Eiichi;
Language: eng
Format: epub
ISBN: 9781788018821
Publisher: Book Network Int'l Limited trading as NBN International (NBNi)
Published: 2019-02-26T16:00:00+00:00

Figure 8.17 The fabrication procedure of immunosensors for the detection of IFN-γ and IL-2. Reproduced from ref. 66 with permission from American Chemical Society, Copyright 2017.

Signal quenching or inhibition strategy is another signal amplification strategy in sandwich type ECL immunosensors. For the ECL quenching principles, Xing et al. constructed a sandwich type ECL immunosensor for the ultrasensitive detection of insulin. 27 As shown in Figure 8.18, luminol was embedded in the matrix that was composited with graphene, SnO2 and Au NPs at first. This composited matrix was used as the ECL probe and sensing platform. Another part of the immunosensor, SiO2 composited with polydopamine (SiO2@PDA), was utilised as an efficient signal quencher and as a label to modify Ab2. In this reported ECL immunosensor, PDA has abundant reactive quinones on its surface for stable anchoring of Ab2 through covalent bonding and Au NPs can highly immobilise Ab1 through the Au–N bond and can further electro-catalyse O2 for the luminol-O2 system. Additionally, the graphene acts as the stable and conductive substrate, which is essential for the construction of the ECL immunosensor. Therefore, such an ECL immunosensor can not only obtain a stable ECL signal but also achieve a highly sensitive detection performance.

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