Future Trends and Challenges of Molecular Imaging and AI Innovation by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9783030927868
Publisher: Springer International Publishing

Figure 2c shows the spectral overlap between UCNP (FRET donor) emission, and mOrange (FRET acceptor) excitation spectrum. We can observe that the broad excitation spectrum of mOrange fluorescent proteins is straddling the green emission band of Cit-UCNPs, satisfying the first condition of FRET. Figure 2d presents the spectroscopic FRET characteristics of UCNP-mOrange nanoprobe. Under the illumination of 980 nm laser, 5 distinct emission band has been observed. Out of these bands, peaks at 520, 521, 540, and 548 nm resembles to the characteristic peaks of UCNPs, and emission peak it 566 nm corresponds to the sensitized emission from mOrange fluorescent proteins. The confirmation of energy transfers between UCNPs and mOrange fluorescent protein is therefore established.

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