Advances in Food Science and Nutrition, Volume 2 by P. M. Visakh;Iturriaga Laura B.;Ribotta Pablo Daniel; & Laura B. Iturriaga & Pablo Daniel Ribotta

Author:P. M., Visakh;Iturriaga, Laura B.;Ribotta, Pablo Daniel; & Laura B. Iturriaga & Pablo Daniel Ribotta
Language: eng
Format: epub
ISBN: 1574374
Publisher: John Wiley & Sons, Incorporated
Published: 2013-11-20T00:00:00+00:00

7.7 Concluding Remarks

In spite of certain discrepancies related to the ‘antioxidant hypothesis’, the message that antioxidants are good for health has considerable momentum worldwide. Today, nutrition and other scientific societies tend to recognize the importance of dietary antioxidants as agents for promoting health and wellbeing. The key nutritional role of foods of plant origin is unquestionable, due to the massive presence of phytochemicals with various biological activities. These plant foods are good sources of major and minor compounds which may have important metabolic and/or physiological effects. More recent evidence provides potential information of their impact on health, so these secondary metabolites are currently marketed as functional foods and nutraceutical ingredients. The authors would like to highlight the fact that there are many methods used to determine total antioxidant activity, and it is important to point out that all of them have some limitations. It has been observed in previous studies that some antioxidant assay methods give different trends. For that reason multiple methods to generate an ‘antioxidant profile’ might be needed.

The most common method to analyse these antioxidants is reversed-phase HPLC with UV detection. This UV detection can be a simple UV-Vis detector or DAD. Various solvents can be used, but are usually selected from an organic phase, ACN or methanol and an aqueous phase, water acidified or not. Identification and quantitation, based mainly on HPLC and LC–MS methods can be aided today by NMR methodology, especially homonuclear two-dimensional correlated spectroscopy, H1–13C heteronuclear multiple-bond correlation spectroscopy and other techniques such as LC-NMR or LC-NMR/MS, which may provide information on the overall composition and enable the identification of individual phenols and caroteniods in complex matrices.

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