Imaging Gene Expression by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781493996742
Publisher: Springer New York

20.Concanavalin A solution (ConA): Dissolve 10 mg of ConA in 10 mL of a solution of 5 mM manganese chloride, 5 mM calcium chloride, and 5 mM Tris–HCl buffer pH 7 in ultrapure water. Store in 1 mL aliquots at −20 °C.

2.3 Consumables

1.Toothpicks to inoculate yeast cultures.

2.Petri dishes for agar plates.

3.PCR tubes.

4.35 mm glass bottom plates for imaging.

5.Focus check beads (e.g., FocalCheck Microspheres or similar).

6.Diffraction limited multicolor fluorescent beads (e.g., TetraSpeck Microspheres or similar).

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