Animal Cell Biotechnology by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781071601914
Publisher: Springer US

9.UltraGlutamine™ is a trade name for the dipeptide l-alanyl-l-glutamine. The dipeptide is used due to its better stability and solubility than l-glutamine. There is less ammonia production during the cultivation and higher antibody titers [20].

10.HT Supplement contains hypoxanthine and thymidine. The pyrimidine and purine derivatives support the biosynthesis of nucleosides.

11.Puromycin is used as selection marker in the CHO-S cell line. The pac gene encoding a puromycin n-acetyltransferase induces a resistance to this antibiotic [21, 22]. During the antibody production process, a medium without puromycin is used to simplify downstream processing.

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