Tissue Engineering by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9783030396985
Publisher: Springer International Publishing

6.8 Cell Storage, Defrosting, and Passaging

Since continuous cell lines are passaged many times, the risk of their genetic instability increases. Therefore, it is very important to keep the stock of these cells in cryogenic storage. Freezing temperatures are not suitable for this purpose, because at regular freezer temperatures (from −20 to −80 °C) the cell viability decreases. For long-term storage of frozen cells, Dewar containers filled with liquid nitrogen are used. They enable maintaining temperatures of −196 °C.

Defrosting cryopreserved cells without significant loss of viability requires quick and careful work. Below are detailed steps for successful thawing (◘ Fig. 6.3):Remove the cryovial containing the frozen cells from liquid nitrogen storage and immediately place it into a 37 °C water bath. It is recommended to cautiously dip the vial in the water bath without exposing the threads or the top lip of the vial to the water to prevent contamination.

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