Processing and Analysis of Biomedical Information by Natasha Lepore & Jorge Brieva & Eduardo Romero & Daniel Racoceanu & Leo Joskowicz

Author:Natasha Lepore & Jorge Brieva & Eduardo Romero & Daniel Racoceanu & Leo Joskowicz
Language: eng
Format: epub
ISBN: 9783030138356
Publisher: Springer International Publishing

Overall Tracking Algorithm. Our procedure for tracking of individual cytoskeletal filaments consists of two main steps: (1) fitting the filament’s position to the image of the current frame and (2) transferring the filament’s curve to the next frame in the sequence. For the second step, we use pyramidal Lucas-Kanade optical flow [2]. It allows to roughly estimate the filament’s position in case of large deformations. Mapping errors are eliminated further on by the repetition of the snakes-based fitting step. In detail, the tracking algorithm consists of the following single steps (see Fig. 1):(A)Initialization: The filament’s position is initialized on the first frame (manually by the user or using an additional segmentation procedure [12]). The endpoint control term is initialized as described in Sect. 3.2;

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