Post-Transcriptional Gene Regulation by Erik Dassi

Author:Erik Dassi
Language: eng
Format: epub, pdf
Publisher: Springer New York, New York, NY

7.Use low adhesion microcentrifuge tubes (e.g., siliconized or DNA LoBind tubes) for all manipulations of the small RNAs after the Proteinase K digestion. The minute amounts of small RNAs to be recovered after RNA isolation will readily adsorb to the walls of standard tubes.

8.It is also possible to use 100 μM of 6-thioguanine (6SG) as the photoactivatable ribonucleoside. 6SG has a lower cross-linking efficiency compared to 4SU and will result in a G-to-A mutation instead of a T-to-C mutation at the cross-linking site.

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