Immuno-Oncology by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781071601716
Publisher: Springer US

5 Assay Optimization

Optimization of bioassays is important to ensure the best possible sensitivity, signal-to-noise ratio, and assay window. The bioassay must also be reproducible, ensuring that small day-to-day variations do not significantly impact the results. Several critical factors during bioassay optimization are considered below.

5.1 Standardizing Assay Reagents

Quality cell culture and assay reagents are critical for consistent bioassay performance. It is prudent to identify reliable suppliers of quality sera, media, and cell culture supplements and establish assay media formulation during bioassay development. Sera, in particular, can have significant impact on cell growth and assay performance depending on grade, region of origin, and supplier. Always anticipate supply constraints and test multiple media and sera suppliers to ensure consistent performance of the bioassay.

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