Plant Protein Secretion by Liwen Jiang

Author:Liwen Jiang
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

3.The time-lapse images are opened in ImageJ software and an ROI is drawn to cover the photobleached region. The values of mean fluorescence intensity are obtained by the analysis tool using the “measure” function in the tool menu for pollen tube images before photobleaching. To measure the fluorescence intensity for the following images, the ROI information is included into ROI manager and named as “ROI1”. Measurement of the values of fluorescence intensity for the following images is performed by clicking “ROI1” and then the “Measure” tool in the “Analyze” menu.

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