Phytochemistry and Pharmacology III by V. K. Singh & J. N. Govil & C. Arunachalam

Author:V. K. Singh & J. N. Govil & C. Arunachalam [Singh, V. K. & Govil, J. N. & Arunachalam, C.]
Language: eng
Format: epub
ISBN: 9781933699073
Google: WNGgXwAACAAJ
Publisher: Studium
Published: 2005-09-15T20:28:43+00:00

CHAPTER-9

Mechanisms for the Antitumor Effect of the

Refined Polysaccharides Extracted fromGanoderma lucidum in Mice

YIHUAI GAO1 AND SHUFENG ZHOU 2*

Abstract

Ganopoly® is an aqueous polysaccharide fraction extracted from G. lucidum by patented biochemical technique and has been marketed as an over-the counter product for chronic diseases including cancer and hepatopathy in many Asian countries. This study was undertaken to explore the antitumor effect and the underlying mechanisms of Ganopoly® in mice and human tumor cell lines. The maximum tolerated dose (MTD) of Ganopoly® in mice was estimated to be 100 mg/kg from a pilot study. Treatment of mice with oral Ganopoly®for 10 days significantly reduced the tumor weight of sarcoma- 180 in a dose-dependent manner, with inhibition rates of32.3, 48.2 and 84.9 % and growth delays of 1.5, 3.5 and 13.1 days at 20, 50 and 100 mg/kg, respectively. Incubation of Ganopoly® at 0.05-1.0 mg/ml for 48 hr showed little or negligible cytotoxicity against human tumor CaSki, SiHa, Hep3B, HepG2, HCT116, HT29, and MCF7 cells in vitro. In contrast, 10 mg/ml of Ganopoly® caused significant cytotoxicity in all tumor cells tested except MCF7, with marked apoptotic effects observed in CaSki, HepG2, and HCT116 cells, as indicated by nuclear staining and DNA fragmentation. In addition, Ganopoly® enhanced concanavalin A-stimulated proliferation of murine

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